High-resolution mucociliary transport measurement in live excised large animal trachea using synchrotron X-ray imaging.

ABSTRACT:

Background

The Australian Synchrotron Imaging and Medical Beamline (IMBL) was designed as the world's widest synchrotron X-ray beam, enabling both clinical imaging and therapeutic applications for humans as well as the imaging of large animal models. Our group is developing methods for imaging the airways of newly developed CF animal models that display human-like lung disease, such as the CF pig, and we expect that the IMBL can be utilised to image airways in animals of this size.

Methods

This study utilised samples of excised tracheal tissue to assess the feasibility, logistics and protocols required for airway imaging in large animal models such as pigs and sheep at the IMBL. We designed an image processing algorithm to automatically track and quantify the tracheal mucoci

SUBMITTER: Donnelley M 

PROVIDER: S-EPMC5434541 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature