Identification of protein targets in cerebral endothelial cells for brain arteriovenous malformation (AVMs) molecular therapies.

ABSTRACT:

Background

To develop a new molecular targeted treatment for brain (AVMs), identification of membrane proteins that are localised on the AVM endothelium is crucial. Current treatment methods are surgery and radiosurgery. However, complete occlusion post radiosurgery are achieved within 3 years, while patient remain at risk of haemorrhage. This study aims to identify potential protein targets in AVM endothelial cells that discriminate these vessels from normal vessels; these proteins targets will be investigated for the molecular therapy of brain AVMs to promote rapid thrombosis after radiosurgery.

Methods

We employed in vitro biotinylation that we developed, and mass spectrometry to detect cell surface-exposed proteins in cultures of murine cerebral endothelial cells (bEnd.3

SUBMITTER: Simonian M 

PROVIDER: S-EPMC5434588 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature