FOXO/DAF-16 Activation Slows Down Turnover of the Majority of Proteins in C. elegans.

ABSTRACT: Most aging hypotheses assume the accumulation of damage, resulting in gradual physiological decline and, ultimately, death. Avoiding protein damage accumulation by enhanced turnover should slow down the aging process and extend the lifespan. However, lowering translational efficiency extends rather than shortens the lifespan in C. elegans. We studied turnover of individual proteins in the long-lived daf-2 mutant by combining SILeNCe (stable isotope labeling by nitrogen in Caenorhabditiselegans) and mass spectrometry. Intriguingly, the majority of proteins displayed prolonged half-lives in daf-2, whereas others remained unchanged, signifying that longevity is not supported by high protein turnover. This slowdown was most prominent for translation-related and mitochondrial proteins. In contr

SUBMITTER: Dhondt I 

PROVIDER: S-EPMC5434875 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature