Laser ablation electrospray ionization high-resolution mass spectrometry for regulatory screening of domoic acid in shellfish.

ABSTRACT:

Rationale

Domoic acid (DA) is a potent neurotoxin that accumulates in shellfish. Routine testing involves homogenization, extraction and chromatographic analysis, with a run time of up to 30 min. Improving throughput using ambient ionization for direct analysis of DA in tissue would result in significant time savings for regulatory testing labs.

Methods

We assess the suitability of laser ablation electrospray ionization high-resolution mass spectrometry (LAESI-HRMS) for high-throughput screening or quantitation of DA in a variety of shellfish matrices. The method was first optimized for use with HRMS detection. Challenges such as tissue sub-sampling, isobaric interferences and method calibration were considered and practical solutions developed. Samples included 189 real she

SUBMITTER: Beach DG 

PROVIDER: S-EPMC5434922 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature