Molecular Cloning, Expression and Peroxidase Conjugation of Staphylococcus aureus Protein A.

ABSTRACT:

Background

Staphylococcal protein A (SPA) is a cell wall component of Staphylococcus aureus that binds to different IgG subclasses of human and several animal species. This bacterial protein can be used as an antibody detector in various immunological assays or as an isolation reagent for the purification of antibody molecules via immuno-chromatography procedures.

Objectives

Molecular cloning and expression of SPA followed by the purification and conjugation of the recombinant protein to peroxidase enzyme.

Material and methods

Encoding DNA fragment of SPA was amplified and inserted into a prokaryotic plasmid vector for the expression of recombinant SPA fused to a maltose binding protein (MBP). The recombinant protein was purified using amylose resin column chro

SUBMITTER: Seyfi Abad Shapouri MR 

PROVIDER: S-EPMC5434992 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature