Cloning and Expression of Iranian Turkmen-thoroughbred Horse Follicle Stimulating Hormone in Pichia pastoris.

ABSTRACT:

Background

Follicle stimulating hormone (FSH) plays an essential role in reproductive physiology and follicular development.

Objective

A new variant of the equine fsh (efsh) gene was cloned, sequenced, and expressed in Pichia pastoris (P. pastoris) GS115 yeast expression system.

Materials and methods

The full-length cDNAs of the efshα and efshβ chains were amplified by reverse transcription polymerase chain reaction (RT-PCR) using the total RNA isolated from an Iranian Turkmen-thoroughbred horse's anterior pituitary gland. The amplified efsh chains were cloned into the pPIC9 vector and transferred into P. pastoris. The secretion of recombined eFSH using P. pastoris expression system was confirmed by Western blott

SUBMITTER: Elyasi Gorji Z 

PROVIDER: S-EPMC5435000 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature