Bacterial Secretome Analysis in Hunt for Novel Bacteriocins with Ability to Control Xanthomonas citri subsp. Citri.

ABSTRACT:

Background

Xanthomonas citri subsp. citri (Xcc), the causative agent of bacterial citrus canker, has affected citriculture worldwide. Varieties of means have been used to minimize its devastating effects, but no attention has been given to bacteriocins.

Objectives

Here and for the first time, we report the isolation and characterization of two novel bacteriocins.

Materials and methods

Secretome containing bacteriocins of isolated bacteria was separated via SDS-PAGE. Each isolated protein band was characterized and checked for its efficacy in controlling two pathogenic isolates of Xcc via disk diffusion assay. The effects of varieties of carbon, nitrogen and phosphate sources were evaluated on both bacterial growth and bacteriocin production via Taguchi o

SUBMITTER: Gholami D 

PROVIDER: S-EPMC5435018 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature