Detection and Characterization of Weissellicin 110, a Bacteriocin Produced by Weissella cibaria.

ABSTRACT:

Background

Weissellicin 110 is the only bacteriocin reported in Weissella cibaria up to now. This bacteriocin represents several unique features. This is the first report on the gene sequence that encodes for the bacteriocin.

Objectives

Providing a rapid detection method to isolate the weissellicin 110 encoding gene and determination of the bacteriocin distribution were the objectives.

Materials and methods

Bacteriocin from W. cibaria 860106 was purified and analyzed using mass spectrometry for proteins sequencing. The draft genome sequence of W. cibaria 860106 was generated using next generation sequencing. PCR was applied to detect the weissellicin 110 encoding gene.

Results

The molecular weight and partial protein sequence were obtained

SUBMITTER: Wu HC 

PROVIDER: S-EPMC5435025 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature