VIT-CMJ2: Endophyte of Agaricus bisporus in Production of Bioactive Compounds.

ABSTRACT:

Background

Agaricus bisporus is an edible basidiomycete fungus. Both the body and the mycelium contain compounds comprising a wide range of antimicrobial molecules, contributing in improvement of immunity and tumor-retardation.

Objectives

The presence of endophytes capable of producing bioactive compounds was investigated in Agaricus bisporus.

Materials and methods

Endophytes from Agaricus bisporus was isolated on LB agar. The obtained isolates were characterized morphologically and biochemically. Further 16S rRNA sequencing was implemented for molecular analysis of isolates. The isolate was mass produced and the bioactive compounds were extracted using ethyl acetate, chloroform and hexane. Agar well diffusion method was carried out to seek the po

SUBMITTER: Gautam CK 

PROVIDER: S-EPMC5435028 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature