ADP-ribosyl cyclases regulate early development of the sea urchin.

ABSTRACT: ADP-ribosyl cyclases are multifunctional enzymes involved in the metabolism of nucleotide derivatives necessary for Ca²⁺ signalling such as cADPR and NAADP. Although Ca²⁺ signalling is a critical regulator of early development, little is known of the role of ADP-ribosyl cyclases during embryogenesis. Here we analyze the expression, activity and function of ADP-ribosyl cyclases in the embryo of the sea urchin - a key organism for study of both Ca²⁺ signalling and embryonic development. ADP-ribosyl cyclase isoforms (SpARC1-4) showed unique changes in expression during early development. These changes were associated with an increase in the ratio of cADPR:NAADP production. Over-expression of SpARC4 (a preferential cyclase) disrupted gastrulation. Our data high

SUBMITTER: Ramakrishnan L 

PROVIDER: S-EPMC5435102 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature