Structural landscape of base pairs containing post-transcriptional modifications in RNA.

ABSTRACT: Base pairs involving post-transcriptionally modified nucleobases are believed to play important roles in a wide variety of functional RNAs. Here we present our attempts toward understanding the structural and functional role of naturally occurring modified base pairs using a combination of X-ray crystal structure database analysis, sequence analysis, and advanced quantum chemical methods. Our bioinformatics analysis reveals that despite their presence in all major secondary structural elements, modified base pairs are most prevalent in tRNA crystal structures and most commonly involve guanine or uridine modifications. Further, analysis of tRNA sequences reveals additional examples of modified base pairs at structurally conserved tRNA regions and highlights the conservation patterns of thes

SUBMITTER: Seelam PP 

PROVIDER: S-EPMC5435857 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature