Single-Molecule Investigations on Histone H2A-H2B Dynamics in the Nucleosome.

ABSTRACT: Nucleosomes impose physical barriers to DNA-templated processes, playing important roles in eukaryotic gene regulation. DNA is packaged into nucleosomes by histone proteins mainly through strong electrostatic interactions that can be modulated by various post-translational histone modifications. Investigating the dynamics of histone dissociation from the nucleosome and how it is altered upon histone modifications is important for understanding eukaryotic gene regulation mechanisms. In particular, histone H2A-H2B dimer displacement in the nucleosome is one of the most important and earliest steps of histone dissociation. Two conflicting hypotheses on the requirement for dimer displacement are that nucleosomal DNA needs to be unwrapped before a dimer can displace and that a dimer can displac

SUBMITTER: Lee J 

PROVIDER: S-EPMC5436051 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature