Drosophila melanogaster positive transcriptional elongation factors regulate metabolic and sex-biased expression in adults.

ABSTRACT:

Background

Transcriptional elongation is a generic function, but is also regulated to allow rapid transcription responses. Following relatively long initiation and promoter clearance, RNA polymerase II can pause and then rapidly elongate following recruitment of positive elongation factors. Multiple elongation complexes exist, but the role of specific components in adult Drosophila is underexplored.

Results

We conducted RNA-seq experiments to analyze the effect of RNAi knockdown of Suppressor of Triplolethal and lilliputian. We similarly analyzed the effect of expressing a dominant negative Cyclin-dependent kinase 9 allele. We observed that almost half of the genes expressed in adults showed reduced expression, supporting a broad role for the three tested genes in steady-sta

SUBMITTER: Yang H 

PROVIDER: S-EPMC5436443 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature