Characterization of an Eukaryotic PL-7 Alginate Lyase in the Marine Red Alga Pyropia yezoensis.

ABSTRACT:

Background

Alginate lyases belonging to polysaccharide lyase family-7 (PL-7) are the most well studied on their structures and functions among whole alginate lyases. However, all characterized PL-7 alginate lyases are from prokaryotic bacteria cells. Here we report the first identification of eukaryotic PL-7 alginate lyase from marine red alga Pyropia yezoensis.

Methods

The cDNA encoding an alginate lyase PyAly was cloned and was used for the construction of recombinant PyAly (rPyAly) expression system in Escherichia coli. Purified rPyAly was assayed to identify its enzymatic properties. Its expression pattern in P. yessoensis was also investigated.

Results

PyAly is likely a secreted protein consisting of an N-terminal signal peptide of 25 residues and a catalytic dom

SUBMITTER: Inoue A 

PROVIDER: S-EPMC5436490 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature