Environmental DNA (eDNA) metabarcoding assays to detect invasive invertebrate species in the Great Lakes.

ABSTRACT: Describing and monitoring biodiversity comprise integral parts of ecosystem management. Recent research coupling metabarcoding and environmental DNA (eDNA) demonstrate that these methods can serve as important tools for surveying biodiversity, while significantly decreasing the time, expense and resources spent on traditional survey methods. The literature emphasizes the importance of genetic marker development, as the markers dictate the applicability, sensitivity and resolution ability of an eDNA assay. The present study developed two metabarcoding eDNA assays using the mtDNA 16S RNA gene with Illumina MiSeq platform to detect invertebrate fauna in the Laurentian Great Lakes and surrounding waterways, with a focus for use on invasive bivalve and gastropod species monitoring. We employed

SUBMITTER: Klymus KE 

PROVIDER: S-EPMC5436814 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature