Electronic cigarette aerosols suppress cellular antioxidant defenses and induce significant oxidative DNA damage.

ABSTRACT:

Background

Electronic cigarette (EC) aerosols contain unique compounds in addition to toxicants and carcinogens traditionally found in tobacco smoke. Studies are warranted to understand the public health risks of ECs.

Objective

The aim of this study was to determine the genotoxicity and the mechanisms induced by EC aerosol extracts on human oral and lung epithelial cells.

Methods

Cells were exposed to EC aerosol or mainstream smoke extracts and DNA damage was measured using the primer anchored DNA damage detection assay (q-PADDA) and 8-oxo-dG ELISA assay. Cell viability, reactive oxygen species (ROS) and total antioxidant capacity (TAC) were measured using standard methods. mRNA and protein expression were evaluated by RT-PCR and western blot, respectively.

Results

SUBMITTER: Ganapathy V 

PROVIDER: S-EPMC5436899 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature