Proteome remodelling by the stress sigma factor RpoS/σ^S in Salmonella: identification of small proteins and evidence for post-transcriptional regulation.

ABSTRACT: The RpoS/σ^S sigma subunit of RNA polymerase is the master regulator of the general stress response in many Gram-negative bacteria. Extensive studies have been conducted on σ^S-regulated gene expression at the transcriptional level. In contrast, very limited information regarding the impact of σ^S on global protein production is available. In this study, we used a mass spectrometry-based proteomics approach to explore the wide σ^S-dependent proteome of the human pathogen Salmonella enterica serovar Typhimurium. Our present goals were twofold: (1) to survey the protein changes associated with the ΔrpoS mutation and (2) to assess the coding capacity of σ^S-dependent small RNAs. Our proteomics data, and complementary assays, unravelled the lar

SUBMITTER: Lago M 

PROVIDER: S-EPMC5437024 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature