De Novo Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case.

ABSTRACT: Zika virus (ZIKV) has recently emerged and is the etiological agent of congenital Zika syndrome (CZS), a spectrum of congenital abnormalities arising from neural tissue infections in utero. Herein, we describe the de novo generation of a new ZIKV isolate, ZIKV_Natal, using a modified circular polymerase extension reaction protocol and sequence data obtained from a ZIKV-infected fetus with microcephaly. ZIKV_Natal thus has no laboratory passage history and is unequivocally associated with CZS. ZIKV_Natal could be used to establish a fetal brain infection model in IFNAR^-/- mice (including intrauterine growth restriction) without causing symptomatic infections in dams. ZIKV_Natal was also able to be transmitted by Aedes aegyp

SUBMITTER: Setoh YX 

PROVIDER: S-EPMC5437134 | biostudies-literature | 2017 May-Jun

REPOSITORIES: biostudies-literature