A rapid and low-cost estimation of bacteria counts in solution using fluorescence spectroscopy.

ABSTRACT: The fluorescence spectrum of bacterially bound acridine orange (AO) was investigated to evaluate its use for the rapid enumeration of bacteria. Escherichia coli ATCC 25922 samples were stained with 2 × 10^-2, 2 × 10^-3 or 2 × 10^-4% w/v AO, followed by 3, 2 or 0 washing cycles, respectively, and fluorescence spectra were recorded using a fibre-based spectroscopic system. Independent component analysis was used to analyse the spectral datasets for each staining method. Bacterial concentration order of magnitude classification models were calculated using independent component weights. The relationship between fluorescence intensity of bound AO and bacterial concentration was not linear. However, the spectral signals collected for AO stain concentration-bacteria

SUBMITTER: Guo R 

PROVIDER: S-EPMC5437196 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature