The fitness costs and trade-off shapes associated with the exclusion of nine antibiotics by OmpF porin channels.

ABSTRACT: The trade-off relationship between antibiotic exclusion and nutrient access across the Gram-negative outer membrane is determined by structural constraints in porin channels. The precise nutritional cost of exclusion is unknown for different antibiotics, as are the shapes of the nutrition-susceptibility trade-off. Using a library of 10 engineered isogenic Escherichia coli strains with structural modifications of OmpF porin expressed at a constant level, susceptibilities were measured for nine antibiotics and the nutritional fitness costs estimated by competitions in chemostats. Different antibiotics exhibited a remarkably varied range of geometries in the nutrition-susceptibility trade-off, including convex, concave and sigmoidal trade-off shapes. The trade-off patterns predict the possibi

SUBMITTER: Phan K 

PROVIDER: S-EPMC5437342 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature