Soil microorganisms can overcome respiration inhibition by coupling intra- and extracellular metabolism: ¹³C metabolic tracing reveals the mechanisms.

ABSTRACT: CO₂ release from soil is commonly used to estimate toxicity of various substances on microorganisms. However, the mechanisms underlying persistent CO₂ release from soil exposed to toxicants inhibiting microbial respiration, for example, sodium azide (NaN₃) or heavy metals (Cd, Hg, Cu), remain unclear. To unravel these mechanisms, NaN₃-amended soil was incubated with position-specifically ¹³C-labeled glucose and ¹³C was quantified in CO₂, bulk soil, microbial biomass and phospholipid fatty acids (PLFAs). High ¹³C recovery from C-1 in CO₂ indicates that glucose was predominantly metabolized via the pentose phosphate pathway irrespective of inhibition. Although NaN₃ prevented ^13<

SUBMITTER: K Bore E 

PROVIDER: S-EPMC5437355 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature