In vitro synthesis of 9,10-dihydroxyhexadecanoic acid using recombinant Escherichia coli.

ABSTRACT:

Background

Hydroxy fatty acids are widely used in food, chemical and cosmetic industries. A variety of dihydroxy fatty acids have been synthesized so far; however, no studies have been done on the synthesis of 9,10-dihydroxyhexadecanoic acid. In the present study recombinant E. coli has been used for the heterologous expression of fatty acid hydroxylating enzymes and the whole cell lysate of the induced culture was used for in vitro production of 9,10-dihydroxyhexadecanoic acid.

Results

A first of its kind proof of principle has been successfully demonstrated for the production of 9,10-dihydroxyhexadecanoic acid using three different enzymes viz. fatty acid desaturase (FAD) from Saccharomyces cerevisiae, epoxide hydrolase (EH) from Caenorhabditis elegance and epoxygenase (EP

SUBMITTER: Kaprakkaden A 

PROVIDER: S-EPMC5437634 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature