Microdissection of the A_h01 chromosome in upland cotton and microcloning of resistance gene anologs from the single chromosome.

ABSTRACT:

Background

Chromosome microdissection is one of the most important techniques in molecular cytogenetic research. Cotton (Gossypium Linnaeus, 1753) is the main natural fiber crop in the world. The resistance gene analog (RGA) cloning after its single chromosome microdissection can greatly promote cotton genome research and breeding.

Results

Using the linker adaptor PCR (LA-PCR) with the primers of rice disease-resistance homologues, three nucleotide sequences PS016 (KU051681), PS054 (KU051682), and PS157 (KU051680) were obtained from the chromosome A_h01 of upland cotton (cv. TM-1). The Blast results showed that the three sequences are the nucleotide binding site-leucine rich repeat (NBS-LRR) type RGAs. Clustering results indicated that they are homologous to

SUBMITTER: Cao X 

PROVIDER: S-EPMC5437636 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature