Globally Optimized Targeted Mass Spectrometry: Reliable Metabolomics Analysis with Broad Coverage.

ABSTRACT: Targeted detection is one of the most important methods in mass spectrometry (MS)-based metabolomics; however, its major limitation is the reduced metabolome coverage that results from the limited set of targeted metabolites typically used in the analysis. In this study we describe a new approach, globally optimized targeted (GOT)-MS, that combines many of the advantages of targeted detection and global profiling in metabolomics analysis, including the capability to detect unknowns, broad metabolite coverage, and excellent quantitation. The key step in GOT-MS is a global search of precursor and product ions using a single liquid chromatography-triple quadrupole (LC-QQQ) mass spectrometer. Here, focused on measuring serum metabolites, we obtained 595 precursor ions and 1 890 multiple reacti

SUBMITTER: Gu H 

PROVIDER: S-EPMC5437843 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature