Increased stem cells delivered using a silk gel/scaffold complex for enhanced bone regeneration.

ABSTRACT: The low in vivo survival rate of scaffold-seeded cells is still a challenge in stem cell-based bone regeneration. This study seeks to use a silk hydrogel to deliver more stem cells into a bone defect area and prolong the viability of these cells after implantation. Rat bone marrow stem cells were mingled with silk hydrogels at the concentrations of 1.0 × 10⁵/mL, 1.0 × 10⁶/mL and 1.0 × 10⁷/mL before gelation, added dropwise to a silk scaffold and applied to a rat calvarial defect. A cell tracing experiment was included to observe the preservation of cell viability and function. The results show that the hydrogel with 1.0 × 10⁷/mL stem cells exhibited the best osteogenic effect both in vitro and in vivo. The cell-tracing experiment shows that cells

SUBMITTER: Ding X 

PROVIDER: S-EPMC5438390 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature