Conferring cellulose-degrading ability to Yarrowia lipolytica to facilitate a consolidated bioprocessing approach.

ABSTRACT:

Background

Yarrowia lipolytica, one of the most widely studied "nonconventional" oleaginous yeast species, is unable to grow on cellulose. Recently, we identified and overexpressed two endogenous β-glucosidases in Y. lipolytica, thus enabling this yeast to use cello-oligosaccharides as a carbon source for growth. Using this engineered yeast platform, we have now gone further toward building a fully cellulolytic Y. lipolytica for use in consolidated bioprocessing of cellulose.

Results

Initially, different essential enzyme components of a cellulase cocktail (i.e,. cellobiohydrolases and endoglucanases) were individually expressed in Y. lipolytica in order to ascertain the viability of the strategy. Accordingly, the Trichoderma reesei endogluc

SUBMITTER: Guo ZP 

PROVIDER: S-EPMC5438512 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature