PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis.

ABSTRACT:

Background

Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity.

Results

The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions reveal

SUBMITTER: Baquero LA 

PROVIDER: S-EPMC5438544 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature