Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues.

ABSTRACT:

Background

Intravascular leukocyte recruitment in most vertebrate tissues is restricted to postcapillary and collecting venules, whereas capillaries and arterioles usually support little or no leukocyte adhesion. This segmental restriction is thought to be mediated by endothelial, rather than hemodynamic, differences. The underlying mechanisms are largely unknown, in part because effective tools to distinguish, isolate, and analyze venular endothelial cells (V-ECs) and non-venular endothelial cells (NV-ECs) have been unavailable. We hypothesized that the atypical chemokine receptor DARC (Duffy Antigen Receptor for Chemokines, a.k.a. ACKR1 or CD234) may distinguish V-ECs versus NV-ECs in mice.

Methods

We generated a rat-anti-mouse monoclonal antibody (MAb) that specifically r

SUBMITTER: Thiriot A 

PROVIDER: S-EPMC5438556 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature