Analysis of variability in high throughput screening data: applications to melanoma cell lines and drug responses.

ABSTRACT: High-throughput screening (HTS) strategies and protocols have undergone significant development in the last decade. It is now possible to screen hundreds of thousands of compounds, each exploring multiple biological phenotypes and parameters, against various cell lines or model systems in a single setting. However, given the vast amount of data such studies generate, the fact that they use multiple reagents, and are often technician-intensive, questions have been raised about the variability, reliability and reproducibility of HTS results. Assessments of the impact of the multiple factors in HTS studies could arguably lead to more compelling insights into the robustness of the results of a particular screen, as well as the overall quality of the study. We leveraged classical, yet highly fl

SUBMITTER: Ding KF 

PROVIDER: S-EPMC5438608 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature