Estimating the Efficiency of Phosphopeptide Identification by Tandem Mass Spectrometry.

ABSTRACT: Mass spectrometry has played a significant role in the identification of unknown phosphoproteins and sites of phosphorylation in biological samples. Analyses of protein phosphorylation, particularly large scale phosphoproteomic experiments, have recently been enhanced by efficient enrichment, fast and accurate instrumentation, and better software, but challenges remain because of the low stoichiometry of phosphorylation and poor phosphopeptide ionization efficiency and fragmentation due to neutral loss. Phosphoproteomics has become an important dimension in systems biology studies, and it is essential to have efficient analytical tools to cover a broad range of signaling events. To evaluate current mass spectrometric performance, we present here a novel method to estimate the efficiency of

SUBMITTER: Hsu CC 

PROVIDER: S-EPMC5438756 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature