RNA-Seq analysis of gene expression for floral development in crested wheatgrass (Agropyron cristatum L.).

ABSTRACT: Crested wheatgrass [Agropyron cristatum L. (Gaertn.)] is widely used for early spring grazing in western Canada and the development of late maturing cultivars which maintain forage quality for a longer period is desired. However, it is difficult to manipulate the timing of floral transition, as little is known about molecular mechanism of plant maturity in this species. In this study, RNA-Seq and differential gene expression analysis were performed to investigate gene expression for floral initiation and development in crested wheatgrass. Three cDNA libraries were generated and sequenced to represent three successive growth stages by sampling leaves at the stem elongation stage, spikes at boot and anthesis stages. The sequencing generated 25,568,846; 25,144,688 and 25,714,194 qualified Ill

SUBMITTER: Zeng F 

PROVIDER: S-EPMC5439701 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature