In silico development and characterization of tri-nucleotide simple sequence repeat markers in hazelnut (Corylus avellana L.).

ABSTRACT: Plant genomes are now sequenced rapidly and inexpensively. In silico approaches allow efficient development of simple sequence repeat markers, also known as microsatellite markers, from these sequences. A search of the genome sequence of 'Jefferson' hazelnut (Corylus avellana L.) identified 8,708 tri-nucleotide simple sequence repeats with at least five repeat units, and stepwise removal of the less promising sequences led to the development of 150 polymorphic markers. Fragments in the 'Jefferson' sequence containing tri-nucleotide repeats were used as references and aligned with genomic sequences from seven other cultivars. Following in silico alignment, sequences that showed variation in number of repeat units were selected and primer pairs were designed for 243 of them. Screening on aga

SUBMITTER: Bhattarai G 

PROVIDER: S-EPMC5439716 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature