Cisplatin induces protective autophagy through activation of BECN1 in human bladder cancer cells.

ABSTRACT:

Purpose

Cisplatin-based chemotherapy is the first line treatment for several cancers including bladder cancer (BC). Autophagy induction has been implied to contribute to cisplatin resistance in ovarian cancer; and a high basal level of autophagy has been demonstrated in human bladder tumors. Therefore, it is reasonable to speculate that autophagy may account for the failure of cisplatin single treatment in BC. This study investigated whether cisplatin induces autophagy and the mechanism involved using human BC cell lines.

Materials and methods

Human BC cells (5637 and T24) were used in this study. Cell viability was detected using water soluble tetrazolium-8 reagents. Autophagy induction was detected by monitoring the levels of light chain 3 (LC3)-II and p62 by Western blot,

SUBMITTER: Lin JF 

PROVIDER: S-EPMC5439993 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature