Bitistatin-functionalized fluorescent nanodiamond particles specifically bind to purified human platelet integrin receptor α_IIbβ₃ and activated platelets.

ABSTRACT: Thromboembolic events (TEE) underwrite key causes of death in developed countries. While advanced imaging technologies such as computed tomography scans serve to diagnose blood clots during acute cardiovascular events, no such technology is available in routine primary care for TEE risk assessment. Here, we describe an imaging platform technology based on bioengineered fluorescent nanodiamond particles (F-NDPs) functionalized with bitistatin (Bit), a disintegrin that specifically binds to the α_IIbβ₃ integrin, platelet fibrinogen receptor (PFR) on activated platelets. Covalent linkage of purified Bit to F-NDP was concentration-dependent and saturable, as validated by enzyme-linked immunosorbent assay using specific anti-Bit antibodies. F-NDP-Bit interacted with purifie

SUBMITTER: Marcinkiewicz C 

PROVIDER: S-EPMC5440039 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature