Universal Solid-Phase Reversible Sample-Prep for Concurrent Proteome and N-Glycome Characterization.

ABSTRACT: We describe a novel solid-phase reversible sample-prep (SRS) platform that enables rapid sample preparation for concurrent proteome and N-glycome characterization for nearly all protein samples. SRS utilizes a uniquely functionalized, silica-based bead that has strong affinity toward proteins with minimal to no affinity for peptides and other small molecules. By leveraging this inherent size difference between proteins and peptides, SRS permits high-capacity binding of proteins, rapid removal of small molecules (detergents, metabolites, salts, peptides, etc.), extensive manipulation including enzymatic and chemical treatments on bead-bound proteins, and easy recovery of N-glycans and peptides. SRS was evaluated in a wide range of samples including glycoproteins, cell lysate, murine tissues

SUBMITTER: Zhou H 

PROVIDER: S-EPMC5440117 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature