Characterizing the lateral friction of nanoparticles on on-chip integrated black lipid membranes.

ABSTRACT: The use of nanoparticles (NPs) in biomedical applications creates a need for appropriate model systems to systematically investigate NP-membrane interactions under well-defined conditions. Black lipid membranes (BLMs) are free-floating membranes with defined composition that are ideally suited for characterizing NP-membrane interactions free of any potential perturbation through a supporting substrate. Herein, arrays of microfabricated BLMs are integrated into a chip-based platform that is compatible with high-speed optical NP tracking. This system is used to investigate the lateral diffusion of 40 nm gold spheres tethered to biotinylated lipids through antibody-functionalized ligands (single-stranded DNA or polyethylene glycol). Although the NPs show an almost free and ergodic diffusion,

SUBMITTER: Chen T 

PROVIDER: S-EPMC5440248 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature