S-nitrosoprotein formation and localization in endothelial cells.

ABSTRACT: Protein S-nitrosation represents a recently described form of post-translational modification that is rapid and reversible. However, the analysis of protein S-nitrosation in situ has been difficult because of the absence of specific probes and the instability of cellular protein S-nitrosothiols. We developed a rapid and specific method for detecting endothelial S-nitrosoproteins patterned after the biotin switch method that involves thiol alkylation followed by reductive generation of thiols from S-nitrosothiols, which are then labeled with either a biotin- or Texas red-derivative of methanethiosulfonate. When we used this methodology, we found that S-nitrosated proteins can form within endothelial cells from an exogenous S-nitrosothiol donor or from endogenous production of NO by endothel

SUBMITTER: Yang Y 

PROVIDER: S-EPMC544053 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature