SNP detection using peptide nucleic acid probes and conjugated polymers: applications in neurodegenerative disease identification.

ABSTRACT: A strategy employing a combination of peptide nucleic acid (PNA) probes, an optically amplifying conjugated polymer (CP), and S1 nuclease enzyme is capable of detecting SNPs in a simple, rapid, and sensitive manner. The recognition is accomplished by sequence-specific hybridization between the uncharged, fluorescein-labeled PNA probe and the DNA sequence of interest. After subsequent treatment with S1 nuclease, the cationic water soluble CP electrostatically associates with the remaining anionic PNA/DNA complex, leading to sensitized emission of the labeled PNA probe via FRET from the CP. The generation of fluorescent signal is controlled by strand-specific electrostatic interactions and is governed by the complementarity of the probe/target pair. To assess the method, we compared the abil

SUBMITTER: Gaylord BS 

PROVIDER: S-EPMC544065 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature