Individualized Breast Cancer Characterization through Single-Cell Analysis of Tumor and Adjacent Normal Cells.

ABSTRACT: There is a need to individualize assays for tumor molecular phenotyping, given variations in the differentiation status of tumor and normal tissues in different patients. To address this, we performed single-cell genomics of breast tumors and adjacent normal cells propagated for a short duration under growth conditions that enable epithelial reprogramming. Cells analyzed were either unselected for a specific subpopulation or phenotypically defined as undifferentiated and highly clonogenic ALDH⁺/CD49f⁺/EpCAM⁺ luminal progenitors, which express both basal cell and luminal cell-enriched genes. We analyzed 420 tumor cells and 284 adjacent normal cells for expression of 93 genes that included a PAM50-intrinsic subtype classifier and stemness-related genes. ALDH<

SUBMITTER: Anjanappa M 

PROVIDER: S-EPMC5441682 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature