The molecular determinants for distinguishing between ubiquitin and NEDD8 by USP2.

ABSTRACT: Ubiquitin (Ub) shares the highest sequence identity with neuronal-precursor-cell-expressed developmentally downregulated protein-8 (NEDD8) in the Ub-like protein family. However, different enzyme systems are precisely employed for targeting Ub and NEDD8 to specific substrates. The molecular determinants for distinguishing between Ub and NEDD8 by Ub-specific peptidases (USPs) remain poorly characterized. By replacing the non-conserved residues of Ub with their NEDD8 equivalents by mutagenesis, and vice versa, we observed that the Ub^4K, Ub^12E, and Ub^14E mutants partially and the Ub^{4K/12E/14E/72A} mutant completely prevented their hydrolysis by USP2. The NEDD8^4F and NEDD8^14T mutants were slightly hydrolyzed by USP2; however, the

SUBMITTER: Shin YC 

PROVIDER: S-EPMC5442100 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature