Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea).

ABSTRACT: In the present study, two elongases, Elovl4 and Elovl5, were functionally characterized and their transcriptional regulation in response to n-3 LC-PUFA administration were investigated in vivo and in vitro. We previously described the molecular characterization of croaker elovl5. Here, we report the full-length cDNA sequence of croaker elovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of coding region that encoded a polypeptide of 302 amino acids possessing all the characteristic features of Elovl proteins. Functional studies showed that croaker Elovl5, displayed high elongation activity towards C₁₈ and C₂₀ PUFA, with only low activity towards C₂₂ PUFA. In contrast, croaker Elovl4 could effectively convert both C₂₀ and

SUBMITTER: Li S 

PROVIDER: S-EPMC5442133 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature