Isolation of biologically active peptides from the venom of Japanese carpenter bee, Xylocopa appendiculata.

ABSTRACT:

Background

Mass spectrometry-guided venom peptide profiling is a powerful tool to explore novel substances from venomous animals in a highly sensitive manner. In this study, this peptide profiling approach is successfully applied to explore the venom peptides of a Japanese solitary carpenter bee, Xylocopa appendiculata (Hymenoptera: Apoidea: Apidae: Anthophila: Xylocopinae: Xylocopini). Although interesting biological effects of the crude venom of carpenter bees have been reported, the structure and biological function of the venom peptides have not been elucidated yet.

Methods

The venom peptide profiling of the crude venom of X. appendiculata was performed by matrix-assisted laser desorption/ionization-time of flight mass spectroscopy. The venom was purified b

SUBMITTER: Kawakami H 

PROVIDER: S-EPMC5442655 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature