Intracellular product recycling in high succinic acid producing yeast at low pH.

ABSTRACT:

Background

The metabolic engineering of Saccharomyces cerevisiae for the production of succinic acid has progressed dramatically, and a series of high-producing hosts are available. At low cultivation pH and high titers, the product transport can become bidirectional, i.e. the acid is reentering the cell and is again exported or even catabolized. Here, a quantitative approach for the identification of product recycling fluxes is developed.

Results

The metabolic flux distributions at two time-points of the fermentation process were analyzed. ¹³C labeled succinic acid was added to the extracellular space and intracellular enrichments were measured and subsequently used for the estimation of metabolic fluxes. The labeling was introduced by a labeling switch experimen

SUBMITTER: Wahl SA 

PROVIDER: S-EPMC5442661 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature