Molecular markers of anti-malarial drug resistance in Central, West and East African children with severe malaria.

ABSTRACT:

Background

The Plasmodium falciparum multidrug resistance 1 (PfMDR1), P. falciparum Ca²⁺-ATPase (PfATP6) and Kelch-13 propeller domain (PfK13) loci are molecular markers of parasite susceptibility to anti-malarial drugs. Their frequency distributions were determined in the isolates collected from children with severe malaria originating from three African countries.

Methods

Samples from 287 children with severe malaria [(Gabon: n = 114); (Ghana: n = 89); (Kenya: n = 84)] were genotyped for pfmdr1, pfatp6 and pfk13 loci by DNA sequencing and assessing pfmdr1 copy number variation (CNV) by real-time PCR.

Results

Pfmdr1-N86Y mutation was detected in 48, 10 and 10% in Lambaréné, Kumasi and Kisumu, respectively. At codon 184, the prevalence of the mutation was 7

SUBMITTER: Nguetse CN 

PROVIDER: S-EPMC5442681 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature