Safety and Efficacy of Megakaryocytes Induced from Hematopoietic Stem Cells in Murine and Nonhuman Primate Models.

ABSTRACT: Because of a lack of platelet supply and a U.S. Food and Drug Administration-approved platelet growth factor, megakaryocytes have emerged as an effective substitute for alleviating thrombocytopenia. Here, we report the development of an efficient two-stage culture system that is free of stroma, animal components, and genetic manipulations for the production of functional megakaryocytes from hematopoietic stem cells. Safety and functional studies were performed in murine and nonhuman primate models. One human cryopreserved cord blood CD34⁺ cell could be induced ex vivo to produce up to 1.0 × 10⁴ megakaryocytes that included CD41a⁺ and CD42b⁺ cells at 82.4% ± 6.1% and 73.3% ± 8.5% (mean ± SD), respectively, yielding approximately 650-fold higher ce

SUBMITTER: Guan X 

PROVIDER: S-EPMC5442772 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature