A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis.

ABSTRACT:

Background

Microbial secondary metabolism is regulated by a complex and mostly-unknown network of global and pathway-specific regulators. A dozen biosynthetic gene clusters for secondary metabolites have been reported in myxobacteria, but a few regulation factors have been identified.

Results

We identified a transcription regulator Esi for the biosynthesis of epothilones. Inactivation of esi promoted the epothilone production, while overexpression of the gene suppressed the production. The regulation was determined to be resulted from the transcriptional changes of epothilone genes. Esi was able to bind, probably via the N-terminus of the protein, to an inverted repeat sequence in the promoter of the epothilone biosynthetic gene cluster. The Esi-homologous sequences retrieve

SUBMITTER: Yue XJ 

PROVIDER: S-EPMC5442856 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature