Deduced probable HLA haplotypes associated with HLA-C*04:82 found by case analysis of Taiwanese individuals.

ABSTRACT:

Objective

HLA-C*04:82 is a low incidence HLA-C allele. The aim here is to report the ethnicity of C*04:82 and its associated HLA haplotypes among Taiwanese individuals.

Materials and methods

A sequence-based typing method was used to confirm this low incidence allele. Polymerase chain reaction was performed to amplify exons 2 and 3 of the HLA-A, HLA-B, and HLA-C loci and exon 2 of the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced in both directions using Terminator Cycle Sequencing Ready Reaction kits and the manufacturer's protocols. The potential unrelated bone marrow stem cell donors in this study were randomized individuals with Taiwanese ethnicity who participated in the Tzu Chi Bone Marrow Donor Registry. The family members in the family

SUBMITTER: Yang KL 

PROVIDER: S-EPMC5442919 | biostudies-literature | 2016 Jul-Sep

REPOSITORIES: biostudies-literature