Diagnostic tool for the identification of MLL rearrangements including unknown partner genes.

ABSTRACT: Approximately 50 different chromosomal translocations of the human MLL gene are currently known and associated with high-risk acute leukemia. The large number of different MLL translocation partner genes makes a precise diagnosis a demanding task. After their cytogenetic identification, only the most common MLL translocations are investigated by RT-PCR analyses, whereas infrequent or unknown MLL translocations are excluded from further analyses. Therefore, we aimed at establishing a method that enables the detection of any MLL rearrangement by using genomic DNA isolated from patient biopsy material. This goal was achieved by establishing a universal long-distance inverse-PCR approach that allows the identification of any kind of MLL rearrangement if located within the breakpoint cluster re

SUBMITTER: Meyer C 

PROVIDER: S-EPMC544299 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature