Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB.

ABSTRACT: In bacteria, double-strand break (DSB) repair via homologous recombination is thought to be initiated through the bi-directional degradation and resection of DNA ends by a helicase-nuclease complex such as AddAB. The activity of AddAB has been well-studied in vitro, with translocation speeds between 400-2000 bp/s on linear DNA suggesting that a large section of DNA around a break site is processed for repair. However, the translocation rate and activity of AddAB in vivo is not known, and how AddAB is regulated to prevent excessive DNA degradation around a break site is unclear. To examine the functions and mechanistic regulation of AddAB inside bacterial cells, we developed a next-generation sequencing-based approach to assay DNA processing after a site-specific DSB was introduced on the c

SUBMITTER: Badrinarayanan A 

PROVIDER: S-EPMC5443536 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature