Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities.

ABSTRACT: Microbial communities metabolize plant biomass using secreted enzymes; however, identifying extracellular proteins tightly bound to insoluble lignocellulose in these microbiomes presents a challenge, as the rigorous extraction required to elute these proteins also lyses the microbes associated with the plant biomass releasing intracellular proteins that contaminate the metasecretome. Here we describe a technique for targeting the extracellular proteome, which was used to compare the metasecretome and meta-surface-proteome of two lignocellulose-degrading communities grown on wheat straw and rice straw. A combination of mass spectrometry-based proteomics coupled with metatranscriptomics enabled the identification of a unique secretome pool from these lignocellulose-degrading communities. Thi

SUBMITTER: Alessi AM 

PROVIDER: S-EPMC5443780 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature